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Feline Immunodeficiency Virus (view source)
Revision as of 13:57, 27 August 2010
, 13:57, 27 August 2010→Laboratory Tests
Diagnosis of FIV is made by demonstrating the presence of antibodies against the virus. ELISA tests are available for in-house use, with some kits detecting antibody to the core protein p24 and others detect antibody to the envelope protein gp4l<sup>2</sup>. Non-haemolysed plasma or serum is used for performing the in-house ELISA. Results must be interpreted with caution.
Diagnosis of FIV is made by demonstrating the presence of antibodies against the virus. ELISA tests are available for in-house use, with some kits detecting antibody to the core protein p24 and others detect antibody to the envelope protein gp4l<sup>2</sup>. Non-haemolysed plasma or serum is used for performing the in-house ELISA. Results must be interpreted with caution.
Once a cat acquires FIV infection, the antibodies created persist for life. This means that an ELISA test at any stage after infection should give a positive result. However, the test has a sensitivity of 98%, and so false positives do occur. Because of this, animals that test positive to an in-house ELISA, should be re-tested using a different test. Laboratories offer an immunoblot (Western blot) to confirm the diagnosis in cats that test ELISA-positive. The problem of using a test that detects anitbodies becomes apparent when it is neccessart to test kittens that are born to an FIV-positive queen.
Once a cat acquires FIV infection, the antibodies created persist for life. This means that an ELISA test at any stage after infection should give a positive result. However, the test has a sensitivity of 98%, and so false positives do occur. Because of this, animals that test positive to an in-house ELISA, should be re-tested using a different test. Laboratories offer an immunoblot (Western blot) to confirm the diagnosis in cats that test ELISA-positive. The problem of using a test that detects anitbodies becomes apparent when it is neccessart to test kittens that are born to an FIV-positive queen. Antibodies against FIV are passively acquired via the milk, and can be detected wi
Passive
Passive
transfer of antibodies occurs via the milk, with the
transfer of antibodies occurs via the milk, with the